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Details· CaCo-2 and Jurkat cell pellets (∼1 × 10 7 cells) were prepare as described above, and extracted for 30 min at 4°C in 1 ml of 50 mM Tris, pH 7.4, 150 mM NaCl, 1% Triton X-100 ± 1 μg/ml filipin and a mixture of protease inhibitors (5 μg/ml each of leupeptin, soybean trypsin inhibitor, and benzamidine; and 1 mM phenylmethylsulfonylfluoride).
DetailsFilipin III is a polyene antibiotic. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form. Also fluorescent cholesterol stain. Technical Data for Filipin III The technical data provided above is for guidance only.
DetailsFilipin III is a polyene antibiotic. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form. Also fluorescent cholesterol stain. Technical Data M.Wt: 654.83 Formula: C 35 H 58 O 11 Solubility: Soluble to 5 mM in DMSO Purity: ≥98% Storage:
DetailsFilipin II | C35H58O10 | CID 101282739 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities ...
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DetailsFilipin binds to cholesterol in membranes, forming ultrastructural aggregates and complexes which can be visualized by freeze- fracture electron microscopy. 1, 2 The binding of cholesterol also decreases the intrinsic fluorescence of Filipin, and this property has also been used to detect cholesterol in membrane fractions. 3
Details· Filipin III 。, Filipin III 。 Ex/Em = 340-380/385-470 nm. …
DetailsNa południu Filipin operują islamistyczne ugrupowania rebelianckie. Dochodzić może do starć z siłami zbrojnymi, a także do porwań cudzoziemców, zarówno na lądzie, jak i na morzu. Na pozostałym obszarze kraju obowiązuje stan wyjątkowy. Spodziewać się należy wzmożonych kontroli i znaczącej obecności sił porządkowych.
DetailsFilipin III Filipin,Filipin 28, S. Quality Control & SDS View current batch: Purity: >90.00% COA (Certificate Of Analysis) Datasheet Cholesterol is both an important structural component of cell membranes and an early intermediate in hormone and bile acid …
Detailswe investigated the effects of inhibitors of clathrin-mediated endocytosis (chlorpromazine and k(+) depletion) and of caveolae-mediated uptake (filipin and genistein) on internalization of fitc-poly-l-lysine-labeled dotap/dna lipoplexes and pei/dna polyplexes by a549 pneumocytes and hela cells and on the transfection efficiencies of these …
DetailsFilipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is …
Details· Filipina, dengan nama resmi disebut sebagai Republik Filipina (bahasa Filipino: Republika ng Pilipinas) adalah sebuah negara kepulauan dan negara kesatuan yang bersistem presidensial dengan berbentuk republik konstitusional di Asia Tenggara, sebelah utara Indonesia, dan timur laut Sabah. Filipina merupakan sebuah negara …
Details· Bezpośrednią przyczyną wojny było więc zajęcie Filipin przez USA w wyniku starcia Amerykanów z Hiszpanami. W styczniu 1899 r. już było wiadome, że USA nie uznają niepodległości kraju i według ustaleń traktatu paryskiego z 10 grudnia 1898 r. zostanie on amerykańską kolonią. W parlamencie Stanów Zjednoczonych zostało to ...
Details· Filipin solution. To prepare 50 mL of filipin solution (0.1 mg/mL), first dissolve 5 mg of filipin powder in 200 μL of dimethyl sulfoxide (DMSO). Add this solution dropwise to 50 mL of PBS 1× under constant vortexing. Keep filipin protected from light. Aliquot this solution and store at −20 °C. 8.
DetailsFilipin binds to cholesterol in membranes, forming ultrastructural aggregates and complexes which can be visualized by freeze- fracture electron microscopy. 1, 2 The binding of …
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Details· Filipíny, plným názvem Filipínská republika, jsou ostrovní stát v jihovýchodní Asii. Rozkládají se na 7641 ostrovech v západním Tichém oceánu. Dělí se na tři geografické celky: Visayské ostrovy, Luzon a Mindanao. S žádným státem nesdílejí pozemní hranici.
Details: Filipin III cas: 480-49-9 : >85% : 654.83 : C35H58O11 :,,。 CAS # 480-49-9 : 16,20,22,24-Octacosapentenoic acid, …
Detailsfilipin staining protocol 1) rinse cells 3x with pbs /pbs 2) fix with 3% paraformaldehyde for 30 minutes at rt 3% 30min 3) rinse cells 3x with pbs pbs 4) incubate with 1 ml of 1.5 mg/ml glycine for 10 minutes at rt, 1ml 1.5mg/ml 10min 5) rinse cells 3x with pbs pbs …
Details2 · Filipiny, oficjalnie Republika Filipin (fil. Pilipinas, Republika ng Pilipinas; hiszp. Filipinas, República de Filipinas; ang. Philippines, Republic of the Philippines) – państwo wyspiarskie w południowo-wschodniej Azji, położone na Archipelagu Filipińskim na Oceanie Spokojnym. Od północy Filipiny oblewane są wodami cieśniny Luzon ...
Details; Filipin III is a cholesterol-binding, fluorescing antibiotic used to detect cholesterol in membranes through freeze fracture electron microscopy CAS: 480-49-9 : >85% …
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DetailsFilipin III is a polyene antibiotic. Binds sterols in fungal membranes. Also inhibits formation of the pathological form of prion protein (PrP-res) from the normal membrane-bound (PrP-sen) form. Also fluorescent cholesterol stain.
Details· filipinstainingprotocolrinsecells3xpbs/pbs3%paraformaldehyde30minutesrt3%30minrinsecells3xpbspbs1.5mg/mlglycine10minutesrt,1ml1.5mg/ml10minrinsecells3xpbspbsstaincellsmlfilipinworkingsolutionrt …
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DetailsFilipin complex is a potent broad spectrum 28-membered ring pentaene macrolide antifungal antibiotic. Filipin complex is also a highly fluorescent polyene. Filipin complex specifically binds free cholesterol and is well …
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